Easy to use Ready to use reagents across whole kit, no need to prepare reagents, only 80 minutes incubation time Wide detection range Detection range between 1.8-1200 pg/mL and low limit of detection up to 1.8 pg/mL Long shelf life 24 months shelf life minimizes lot changes, which ensures long term consistency and precision of results Automation ready Simple procedure is automatable on most robotic platforms
Reagents Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with 25-Hydroxyvitamin D derivant
• Calibrators, 6 vials, 1 mL of each, ready to use; Concentrations: 0(A), 7.5(B),
• 15(C), 30(D), 75(E) and 150(F) ng/ml
• Incubation Buffer, 1 vial, 6.0 mL. Contains 0.2% ProClin300 preservative.
• Enzyme Conjugate, Ready to use, 1 vial, 6.0 mL of HRP (horseradish peroxidase) labeled sheep monoclonal Anti-25-Hydroxyvitamin D in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.2% ProClin300 preservative. • Substrate, 1 vial, 11mL, ready to use, (tetramethylbenzidine) TMB
• Stop Solution, 1 vial, 6.0 mL of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 mL (40X concentrated), PBS-Tween
• IFU, 1 copy
• Plate Lid: 1 piece.
Storage
• Store at 2-8℃
• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date, whichever is earlier.
• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at -20 °C. Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled
