Anti Ds-DNA IgG ELISA Test Kit
Drug Names
Generic Name:Anti Ds-DNA IgG ELISA Test Kit
INTENDED USE
Anti-ds-DNA IgG ELISA Test Kit (Serum/Plasma)
INTENDED USE
This kit is a qualitative detection of human serum/plasma of Anti-double Stranded DNA antibody. The kit is suitable for clinical screening and diagnosis. Detection of ds-DNA antibodies is very important for the diagnosis and treatment of lupus erythematosus (SLE). Patients with other connective tissue diseases can also get positive result for anti ds-DNA, and the disease is generally SLE overlap syndrome. Therefore, anti ds-DNA antibody is one of the criteria for the diagnosis of lupus erythematosus.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
PRINCIPLE
This kit uses indirect ELISA principle to detect ds-DNA antibody. Purified ds-DNA antigen is pre-coated on the microplate, the ds-DNA antibody in sample will combine with ds-DNA antigen first, then combine with enzyme-labeled second antibody to form antigen-antibody-anti-antibody complex, and shows blue color in the microplate. This kit is used for the specific detection of ds-DNA antibody in human serum/plasma.
Materials provided with the kit:
| Components | 96T/Box | 480T/Box |
| Coated Microtiter Plate | 1 bag | 8*12 | 5 bags | 8*12 |
| Conjugate | 1 vial | 6.5 mL | 5 vials | 6.5 mL |
| Wash Buffer (40X) | 1 vial | 20 mL | 5 vials | 20 mL |
| Sample Diluent | 1 vial | 11 mL | 5 vials | 11 mL |
| Substrate A | 1 vial | 7 mL | 5 vials | 7 mL |
| Substrate B | 1 vial | 7 mL | 5 vials | 7 mL |
| Stop Solution | 1 vial | 6 mL | 5 vials | 6 mL |
| Cut-off Control | 1 vial | 1 mL | 5 vials | 1 mL |
| Positive Control | 1 vial | 1 mL | 5 vials | 1 mL |
| Closure Plate Membrane | 3 sheets | | 15 sheets | |
Note: different batches of reagent kit, and different component cannot be exchanged for use. Once open, stable for 3 months at 2-8°C.
Important notes
- The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature then use, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
- washing buffer will Crystallization separation, it can be heated the water helps dissolve when
dilute . Washing does not affect the result.
- add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
- Please make specification curve when you assay, had better make duplicate well, if in the sample the testing material content is excessively high (The sample OD is bigger than the first standard well OD ),please use Sample dilution to dilute certain multiple (n times),then assay. Please multiply total Dilution Times when calculate(×n×5).
- Closure plate membrane only limits the disposable use, in order to avoid the overlapping pollution.
- The substrate please evade the light preservation.
- Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard .
- All samples, washing buffer and each kind of reject should according to infective material process.
- This reagent which different batch number component do not mix.
- If it’s different form English instruction, take English instruction as the standard.
Storage and stability
• Store at 2-8℃.
• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
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Anti Ds-DNA IgG ELISA Test Kit Qualitative Anti Double Stranded DNA Antibody Images
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