Product Description:
The thyroid gland is essential for the development of many tissues and for the adjustement of their metabolism. These processes are under the control of thyroid hormones, T4 and T3.
Under stimulation by pituitary thyrotropin(TSH). the thyroid gland essentially secretes T4 with minute amounts of T3.
Tissue recptors to thyroid hormones are T3 receptors, T3 represents thus the acive entity among thyroid secretions.
The bulk of circulating T3 is produced by peripheral deiodination of T4 alternatively, T4 yields reverse T3(or RT3), a poorly active thyronine.
In the blood,T3 circulates free and bound to the same carrier protein transporting T4, thyroxine binding globulin or TBG. Nevertheless, T3 binding affinity to TBG is significantly less than that of T4; The free T3 serum concentration represents about 0.3% of the total circulating T3 concentration.
Under normal conditions, total T3 level is negatively. Correlated to serum TSH level due to the negative feed -back mechanism regulating thyroid funcion.
Features:
- Product Name: T3 Elisa Test Kit
- Specimen: Serum 50μl
- Sensitivity: High
- Storage Temperature: 2-8°C
- Kit Size: 96 Tests
- Test Type: ELISA
- T3 kit is based on competitive method. Coat micro well plates with anti-T3 to form solid-phase antibody. The T3 of sample and biotin-T3 compet with anti-T3 on solid-phase. Vidin-HRP added, solid phase antibody-biotinylated T3-avidin-HRP complex was formed. Add substrate and detect absorbent value. Calculate T3 content of patient serum through computer or plotting fitting concentration-absorbent value curve.
Related Tips:
- This kit is designated for In-Vitro Diagnostic Use Only.
- Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 3 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all wash buffer out and block the rim of well on absorbent paper for a few seconds. Filling each well with water and remain 10 seconds. Repeat these steps 3 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
- Drip procedure. Mix the bottle gently before use. Violent surge may cause too much foam. Invert the bottle and squeeze one or two drop on absorbent tissue to make sure there is no foam. Take the bottle upright the well and make sure the drop does not touch the rim of wells.
- Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.
- Storage. The whole kit should be stored at 2~8℃ for one year. Microplate should be taken to room temperature before opened. This is very important because absorbed atmospheric moisture by cold plates significantly reduces their shelf life. After removing the required number of strips, the plate should be put in the plastic resealable bag with
desiccants to minimize exposure to damp air.
- Control serum is prepared with human serum, which is tested negative of HBV, HCV and HIV. But it should still be considered as capable of transmitting viral diseases.
Packing and Shipping:
Product Packaging:
- Elisa Test Kit
- User Manual
- Buffer Solution
- Reagents
Shipping:
- Shipped by air
- Shipping calculated at checkout
- Estimated delivery time is 3-5 business days
