High accuracy Prolactin ELISA TEST KIT

INTENDED USE

The Prolactin assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of prolactin in human serum. For professional use only.

Test principle

Sandwich principle.

Total duration of assay: 80 minutes.

• Sample, Anti-Prolactin coated microwells and enzyme labeled Anti-Prolactin are combined.

• During the incubation, prolactin presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the prolactin molecules being sandwiched between the solid phase and enzyme-linked antibodies.

• After washing, a complex is generated between the solid phase, the prolactin within the sample and enzyme-linked antibodies by immunological reactions.

• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.

• The absorbance is proportional to the amount of Prolactin in the sample.

Specimen collection and preparation

• Human serum is recommended for this assay.

• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7 days at 2-8℃, and 1 month at -20℃. Freeze only once.

• Do not use heat-inactivated samples.

• Sediments and suspended solids in samples may interfere with the test result which should be removed by centrifugation. Ensure that complete clot formation in serum samples has taken place prior to centrifugation.

• Avoid grossly hemolytic, lipemic or turbid samples.

Storage and stability

• Store at 2-8℃.

• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.

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